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Molecular Neurobiology BioNB 430

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Meeting Times: The lab meets on Tuesdays at 9AM in 302 Wing Hall. The lab lasts most of the day but you can go to other classes on Tuesdays if the class meets after 11AM. There is a Thursday section that will be scheduled so that everyone can attend.

Syllabus

Lab Topic

Lab 1- Enhancer traps and reporter genes

Lab 2 - NMDA receptor cDNA isolation Part I

Lab 3 - NMDA receptor cDNA isolation Part II

Lab 4 - NMDA receptor cDNA isolation Part III

Lab 5- NMDA receptor cDNA isolation Part IV

Lab 6 Western Blotting of Mutant Fly Head Extracts Part I

Lab 7 Western Blotting of Mutant Fly Head Extracts Part II

Lab 8 Immunoprecipitation of Proteins Part I

Lab 9 Immunoprecipitation of Proteins Part II

Lab 10 GST fusion protein purification and pulldown Part I

Lab 11 GST fusion protein purification and pulldown Part II

Lab 12 GST fusion protein purification and pulldown Part Part III

Lab 13 GST fusion protein purification and pulldown Part Part IV